Color → additive → what it's for
- Light blue: sodium citrate: coagulation studies (PT/INR, aPTT). Fill to the line; the blood-to-additive ratio matters.
- Red: no additive (or a clot activator in plastic tubes): serum for many chemistry, serology, and blood-bank tests.
- Gold / SST ("tiger top"): clot activator + gel separator: serum chemistry; the gel separates serum from cells after spinning.
- Green: heparin (lithium or sodium): plasma chemistry, stat chemistries, and ammonia.
- Lavender / purple: EDTA: CBC and most hematology; also HbA1c.
- Gray: sodium fluoride + potassium oxalate: glucose and lactate (fluoride preserves glucose).
- Yellow: two kinds: SPS for blood cultures, or ACD for DNA/HLA testing.
Exact tube choices, brands, and any facility-specific rules live in your laboratory's test directory.
Which tube for common tests
- CBC → lavender (EDTA).
- CMP / BMP → gold/SST (serum), or green (lithium heparin) at some labs.
- PT/INR → light blue (sodium citrate).
- HbA1c → lavender (EDTA) at many labs.
- Glucose (preserved) → gray (fluoride/oxalate).
When a test could go in more than one tube, your lab's directory decides: check it.
Handling & inversions
Additive tubes need gentle inversions right after they fill so the additive mixes and the specimen doesn't clot, but the number of inversions varies by tube type and manufacturer. Follow each tube's instructions for use and your facility's guidance; don't shake.
See every tube in the app
Phlebotomy Toolkit's Test & Tube reference shows each test's tube color, additive, handling, inversions, and common rejection risks: searchable and offline.
$9.99 · one-time · no subscription · iPhone & iPad